Analysis of the complete genomic sequence of an isolate of Tobacco vein banding mosaic virus from Yunnan

The virus was first observed from Potyvirus with electron microscope. The virus was then identified as TVBMV by RT-PCR using primers M4 and Sprimers. The primers were designed using the genomic sequence of the 3' end of Potyvirus. The isolate was named YN-YX-TVBMV. A 6047 bp sequence was obtained from eight segments of YN-YX-TVBMV, This sequence was used to compare with TVBMV isolates from China. The results indicated that significant sequence variation existed among TVBMV isolates from diferent locations with the isolates from Yunnan Province always formed a separate group.