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    DING Gensheng, GE Yonghui, YANG Chao, LI Yongqing, WANG Chengfu, HUANG Xinmin, HUANG Zhenghong, YANG Shenggang, WAN Lixing, HUI Jianquan, GENG Zhaoliang. Antioxidant Activity and Active Component Identification of Glycosides from Leaves of Guizhou Flue-cured Tobacco[J]. CHINESE TOBACCO SCIENCE, 2017, 38(5): 87-92. DOI: 10.13496/j.issn.1007-5119.2017.05.015
    Citation: DING Gensheng, GE Yonghui, YANG Chao, LI Yongqing, WANG Chengfu, HUANG Xinmin, HUANG Zhenghong, YANG Shenggang, WAN Lixing, HUI Jianquan, GENG Zhaoliang. Antioxidant Activity and Active Component Identification of Glycosides from Leaves of Guizhou Flue-cured Tobacco[J]. CHINESE TOBACCO SCIENCE, 2017, 38(5): 87-92. DOI: 10.13496/j.issn.1007-5119.2017.05.015

    Antioxidant Activity and Active Component Identification of Glycosides from Leaves of Guizhou Flue-cured Tobacco

    • To explore the chemical constituents of tobacco glycosides and their antioxidant activities, crude glycoside extracts from Guizhou flue-cured tobacco leaves were obtained by methanol-reflux and macroporous resin adsorption. DPPH radical-scavenging activities of different extracts of crude glycoside were investigated. Further separation and purification of ethyl acetate fractions were processed through reversed phase chromatography, gel chromatography, preparative liquid chromatography and thin layer chromatography. The results showed that, each crude glycoside extract showed certain DPPH radical-scavenging activities, in which the ethyl acetate fraction exhibited the best activity. Multiple monomeric compounds were isolated from the ethyl acetate fraction, and four glycoside chemical compounds were identified by NMR. The four monomers are 7,8-dihyd-3-oxo-α-ionol-β-Dglucopyranoside(1), 3-oxo-α-ionol-β-D-glucopyranosid (2), 6-O-β-D-glucopyranosid(3) and O-β-D-glucopyranosid(4).
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