Construction of Tobacco Virus Resistance-related R Genes RNAi Expression Vectors

Gene silence mediated by RNA interference has been shown to be a highly rapid and effective method for breeding resistance to virus and analyzing plant functional genomics. Building of RNAi expression vector and being transformed into tobacco can obtain virus resistance tobacco materials and provide a new method for identification of tobacco functional genes in a large-scale. The partial sequence of R genes with specific banding site attB were amplified by PCR technology, primers were designed according to the sequences obtained from Nicotiana tomentosiformis sequencing. Using GATEWAY technology, the RNAi expression vector of R gene was constructed successfully by insered the partial sequence of R genes into the vector of pH7GWIWG2(I).