BAC Library Construction of Villi Tobacco (<i>Nicotiana tomentosiformis</i>)

GAO Xiaoming; CHEN Yanling; LIU Guanshan; LI Fengxia; WANG Weifeng; REN Yuanyuan; SUN Yuhe

doi:10.3969/j.issn.1007-5119.2012.03.014

GAO Xiaoming, CHEN Yanling, LIU Guanshan, LI Fengxia, WANG Weifeng, REN Yuanyuan, SUN Yuhe. BAC Library Construction of Villi Tobacco (Nicotiana tomentosiformis)[J]. CHINESE TOBACCO SCIENCE, 2012, 33(3): 68-71. DOI: 10.3969/j.issn.1007-5119.2012.03.014

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BAC Library Construction of Villi Tobacco (Nicotiana tomentosiformis)

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Villi tobacco (Nicotiana tomentosiformis) is an important species of wild tobacco. The BAC library was constructed with CopyControl^TM pCC1BAC^TM (HindⅢ Cloning-Ready) Vector. From the analysis of randomly selected BAC clones, the inserted DNA size of BAC library ranged from 50 to 200 kb; the average estimated was 110 kb; the empty clone was less than 2%; the insert was stable in a hundred generations.

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BAC Library Construction of Villi Tobacco (Nicotiana tomentosiformis)

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