Medium Optimization of Antagonistic Bacteria A3 Strain Against TMV

The experiments with one factor and orthogonal design were conducted to optimize medium of antagonistic bacteria A3 strain against TMV (Pseudomonas putida) with mass growth (OD₆₀₀ value) as the index. A systematic study of carbon sources, nitrogen sources and inorganic chemicals was carried out. Carbon source “wheat bran”, nitrogen source “yeast extract powder” and inorganic chemical “CaCl₂, CoCl₂, MgSO₄, FeSO₄, KH₂PO₄”, were beneficial to the growth of A3. The results of L₁₈(3⁷) orthogonal tests showed that optimal medium menu included 15g wheat bran,15g yeast extract powder, 2.5g CaCl₂, 0.5g CoCl₂, 0.8g MgSO₄, 0.5g FeSO₄, 0.8g KH₂PO₄ and 1000mL distilled water, pH 7.0. The number of live bacteria could be 3.234×10¹² cfu/mL in the optimized medium and the number increased by one orders of magnitude than original NB medium.