Abstract: To determine the control efficacy of spraying S-methoprene against tobacco beetle (Lasioderma serricorne) and its impact on the quality of tobacco leaves during stripping and re-drying process, laboratory tests were conducted to detect the biological activity of various concentrations of S-methoprene sprayed on tobacco strips against tobacco beetle, and S-methoprene was sprayed using specialized sprayer during leaf stripping and re-drying process for analyzing control efficacy against tobacco beetle, as well as the chemical compositions and sensory quality of tobacco strips. Results showed as the follows. (1) The emergence rates of tobacco beetle reached 7.78% when the concentration of S-methoprene was 1.25 mg/kg, while the emergence rates exhibited zero when the concentration was over 5 mg/kg. (2) Seven days after the spraying of 15 mg/kg S-methoprene on the leaf stripping and re-drying line, the emergence rate of tobacco beetle introduced averaged only 2.49% (compared to 93.33% in the control group), and one year after the storage of flue-cured tobacco treated with S-methoprene, the average emergence rate was 3.07%. (3) The inhibitory efficacy of S-methoprene sprayed on the leaf stripping and re-drying line against adult tobacco beetle was over 95%. (4) Spraying 15 mg/kg S-methoprene on leaf stripping and re-drying line had no significant impact on the chemical compositions and sensory quality of tobacco leaves. The application of S-methoprene via spraying on the leaf stripping and re-drying line can effectively control tobacco storage pests.