Abstract: Plant growth-promoting rhizobacteria (PGPR), a group of microorganisms exhibiting remarkable growth-promoting and biocontrol activities, can enhance soil nutrient availability, promote plant development, and confer protection against plant diseases and insect pests through nitrogen fixation, solubilization of phosphorus and potassium, biosynthesis of plant hormones, and production of siderophores. Mechanisms by which plant growth-promoting rhizobacteria influence plant growth and development through direct or indirect effects are comprehensively reviewed in this paper. The potential and advantages of plant growth-promoting rhizobacteria in promoting tobacco (Nicotiana tabacum L.) growth via multiple mechanisms, including nitrogen fixation, phosphorus solubilization, potassium dissolution, auxin synthesis, and alleviation of abiotic stress, are analyzed. Furthermore, the research status of practically applied plant growth-promoting rhizobacteria in flue-cured tobacco, classified based on functions such as nutritional growth promotion and disease and pest resistance, is discussed. Meanwhile, key issues and corresponding countermeasures regarding the application of plant growth-promoting rhizobacteria in tobacco are summarized. Finally, the current challenges and future development trends of PGPR are prospected, aiming to provide references for tobacco production and disease control.