Abstract: In order to investigate the relationship between quality of flue-cured tobacco and soil texture, 170 tobacco leaf samples and 170 soil samples of four types of soil texture were studied. The results showed the majority of tobacco planting soils in Guizhou province had texture of middle and moderately clay. Tobacco leaves planted on sandy loam was with characteristic of high sugar, low nicotine, high ratio of sugar to nicotine and high ratio of all nitrogen to nicotine. Tobacco leaves planted on silty clay was with characteristic of low sugar, high nicotine, low ratio of sugar to nicotine and low ratio of all nitrogen to nicotine. Because of suitable soil fertility and about 7% volume of gravelly in soil texture of high clay, the sensory evaluation score of tobacco leaf planted on heavy soil was better than those planted on light soil.