绒毛状烟草基因组中SSR位点的信息分析

Analysis of SSR Information in N.tomentosiformis Goodspeed genome

摘要: 通过SSRFINDER软件搜索绒毛状烟草基因组DNA，设置条件是重复单元长度2~5 bp，重复次数10～60，共找到6 441个SSR位点，涉及5 227个Scaffold，SSR序列总长为303 963 bp，约占全基因组的0.12‰，即绒毛状烟草基因组中平均每400 kb会出现一个SSR位点。在所有SSR位点中，二、三碱基重复基元分别占总数的45.2%和49.6%，重复单元的重复次数10～20之间的SSR位点占总数的74.6%。

Abstract: By searching N. tomentosiformis Goodspeed genome DNA with SSRFINDER, 6441 SSRs were identified from 5227 scaffold. Total length 303 963bp of SSRs occupied approximately 0.12‰ of genome DNA. The average distance between SSRs was about 400 kb. Dinucleotide and trinucleotide motifs were most abundant classes, 45.2% and 49.6% respectively. The repeats of motifs from 10-20 accounted for 74.6% of the total SSRs.