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    熊敏, 苗朝棋, 余涓萍, 王冬, 马昕, 谭本奎, 陈振国, 陈守文. 饼粕肥对芽胞杆菌在烟草根际定殖及促生作用的影响[J]. 中国烟草科学.
    引用本文: 熊敏, 苗朝棋, 余涓萍, 王冬, 马昕, 谭本奎, 陈振国, 陈守文. 饼粕肥对芽胞杆菌在烟草根际定殖及促生作用的影响[J]. 中国烟草科学.
    XIONG Min, MIAO Zhaoqi, YU Juanping, WANG Dong, MA Xin, TAN Benkui, CHEN Zhenguo, CHEN Shouwen. Effects of cake fertilizer on rhizosphere colonization of Bacillus and growth promotion of tobacco[J]. CHINESE TOBACCO SCIENCE.
    Citation: XIONG Min, MIAO Zhaoqi, YU Juanping, WANG Dong, MA Xin, TAN Benkui, CHEN Zhenguo, CHEN Shouwen. Effects of cake fertilizer on rhizosphere colonization of Bacillus and growth promotion of tobacco[J]. CHINESE TOBACCO SCIENCE.

    饼粕肥对芽胞杆菌在烟草根际定殖及促生作用的影响

    Effects of cake fertilizer on rhizosphere colonization of Bacillus and growth promotion of tobacco

    • 摘要: 为了探究饼粕肥对芽胞杆菌在烟草根际定殖的作用及定殖后芽胞杆菌对烟苗的促生效应,在烟苗种植土中分别添加解淀粉芽胞杆菌HZ13(P1)、饼粕肥(P2)、饼粕肥与HZ13即时混合物(P3)及饼粕肥与HZ13共发酵培养物(P4),以无添加的烟苗种植土为对照(CK)。提取各处理烟苗根际代谢物并分析组间差异代谢物,比较不同处理对HZ13-RFP的趋化作用、烟苗根际微生物数量及烟苗生长指标的影响。结果表明,P4根际代谢物种类最多,且有机酸数量最多;P4和CK的差异代谢物20种,以有机酸和糖类为主,含量最丰富的有葡萄糖和麦芽糖,和P1的差异代谢物26种,有机酸和糖类最多,含量最丰富的有麦芽糖、鹅去氧胆酸、琥珀酸、海藻糖、果糖和苏氨酸,和P3的差异代谢物23种,有机酸最多,含量最丰富的有琥珀酸、鹅去氧胆酸和苏氨酸。P4根际代谢物对HZ13-RFP的趋化性及促生作用最强。烟苗移栽后40 d,P4根际HZ13总菌数较P3高12.15倍,占根际细菌总数的12.12%,而P1为0。P4烟苗最大叶面积较P3、P1和CK分别提高30%、140%和160%,株高较P3、P1和CK分别提高100%、230%和300%。综上,饼粕肥与芽胞杆菌共发酵培养对芽胞杆菌在烟苗根际定殖及促生作用发挥效果最好。

       

      Abstract: In order to investigate the effect of cake fertilizer on the rhizosphere colonization of Bacillus and the growth promotion impact of colonized Bacillus on tobacco seedlings, Bacillus amylolyticus HZ13 (P1), cake fertilizer (P2), instantaneous mixture of cake fertilizer and HZ13 (P3), and co-fermentation culture of cake fertilizer and HZ13 (P4) were added to tobacco seedling soil respectively, and soil without any additives served as the control (CK). The rhizosphere metabolites of tobacco seedlings in each treatment were extracted and the different metabolites between treatments were analyzed. The chemotaxis of HZ13-RFP, the number of rhizosphere microorganisms and the growth index of tobacco seedlings under different treatments were compared. The results showed that P4 rhizosphere had the most metabolites and organic acids. There were 20 differential metabolites between P4 and CK, mainly organic acids and saccharides, with glucose and maltose being the most abundant. 26 differential metabolites were found between P4 and P1, mainly organic acids and saccharides, of which, the most abundant ones are maltose, chenodeoxycholic acid, succinic acid, trehalose, fructose and threonine. There were 23 differential metabolites between P4 and P3, mainly organic acids, with succinic acid, chenodeoxycholic acid and threonine being the most abundant. P4 rhizosphere metabolites had the strongest chemotactic and growth-promoting effects on HZ13-RFP. 40 days after transplanting of tobacco seedlings, the count of HZ13 in P4 rhizosphere was 12.15 times higher than that in P3, accounting for 12.12% of the total rhizosphere bacteria, while P1 was 0. Compared with P3, P1 and CK, the maximum leaf area of seedlings was increased by 30%, 140% and 160% in P4, and plant height by 100%, 230% and 300%, respectively. In conclusion, the co-fermentation of cake fertilizer and Bacillus showed the best effects on the rhizosphere colonization of Bacillus and growth promotion of tobacco seedlings by Bacillus.

       

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