Abstract: The effects of stable time of temperature at 54 ℃on the quality and output value of flue-cured tobacco were investigated in order to improve curing efficiency and tobacco quality. The results showed that the curing treatment with gradually yellowish tobacco under soft temperature at yellowing stage, constant temperature at 54 ℃ for 16 h for upper leaves, 24 h at 54 ℃ for middle leaves, and 8 h or 16 h at 54 ℃ for lower leaves, could increase aroma precursors content of the flue-cured tobacco. The appearance quality of the tobacco leaf were relatively high with constant temperature at 54 ℃ for 16h for upper leaves, 8 h or 24 h at 54 ℃ for middle leaves, and 16 h at 54 ℃ for lower leaves in the curing process. Physical indicators were better for constant temperature at 54 ℃ for 16h or 24 h for lower leaves, 8 h at 54 ℃ for middle leaves, 16 h at 54 ℃ for upper leaves in the curing process. The smoking aroma quality significantly increased as well. The taste was pure and mild, and smoke concentration, offensive odor, irritancy and strength were low when constant temperature was at 54 ℃ for 8 h or 16 h for lower leaves, 8 h at 54 ℃ for middle leaves, 16 h or 24 h at 54 ℃ for upper leaves. An integrated analysis of the quality and economic values of tobacco leaves for different treatments showed that the better comprehensive quality and economic values of tobacco leaves were obtained when the curing treatment of gradually yellowish tobacco were under soft temperature at yellowing stage, and constant temperature at 54 ℃ for 16 h for lower leaves with proper maturity, 8 h at 54 ℃ for middle leaves with maturity, 16 h at 54 ℃ for upper leaves with maturity.