Abstract: In order to further understand the growth characteristics of Guizhou flue-cured tobacco, the difference of agronomic attributes and photosynthetic characteristics in different growth stages (35, 70, 105 d after transplanting) with K326, Bina 1, Guiyan 1, Guiyan 2 and organic tobacco K326 as material were studied. The result showed that Guiyan 2 had better agronomic attributes in field growth stage, and dry weight was 349.46 g/plant on 105 d after transplanting, and Bina 1 was 323.59 g/plant. Photosynthetic characteristic parameters of Guiyan 2 were the best, which could be used as potential material for variety breeding, then the next were K326 and Guiyan 1. The chlorophyll content of K326 on 35 d and 70 d was 35.72 and 46.75, while Bina 1 was 47.32 on 70 d after transplanting. Both agronomic attributes and photosynthetic characteristics of organic tobacco K326 were worse. However, they were easy to cured. Agronomic attributes and photosynthetic characteristics and the content of chlorophyll were good judgment indices for guiding tobacco production, meanwhile they had a close relationship with variety breeding, curing characteristic and tobacco quality.