Abstract: The main chemical constituents of 105 leaf samples of flue-cured tobacco (cv. Honghuadajinyuan) from 3 counties in Panzhihua were analyzed to provide scientific basis for comprehensive quality evaluation. The main chemical constituents and ratio of tobacco leaves was comprehensive evaluated by factor analysis. The results showed that the main chemical constituents of tobacco leaves in Panzhihua were harmonious in general. The contents of total sugar and reducing sugar were relatively high, but contents of potassium were relatively low. There was a significant negative correlation between the nitrogen compounds and carbon containing compound, and there was a significant positive correlation between the total nitrogen and total alkaloid in the main chemical constituents of the tobacco leaves. Three main factors from the main chemical constituents and derivative composition were obtained by factor analysis, representing 80% of total variance. The contribution rate of carbon nitrogen factor was the highest and the factor of combustion took second place. The application rates of nitrogen, potassium fertilizers and green manure need to be controlled in tobacco field production, so that the ratio of every chemical constituents in flue-cured tobacco can be coordinated.