Abstract: The Yunbai 3 is a new burley tobacco variety by pedigree selection. It was sent to agriculture accreditation of national new tobacco varieties in 2010, and passed the validation in 2012. It grew tidy in field and had stronger growth vigor with more effective leaves and excellent agronomic preperties. The variety has better fertilizer tolerance and adaptation. Yunbai 3 is high resistant to TMV, resistant to black shank, less sensitive to south root knot nematode, sensitive to brown spot. The main economic characters were fine. The regression analysis of the environment interaction effects in national variety test and stability showed that, the yield and output value had good stability and high yielding in four provinces. The overall appearance quality and physical characteristics of the leaves were slightly better than those of control variety. Harmony of chemical composition and sensory quality were close to those of the control variety.