Abstract: To accomplish the target of "reducing the labor and cost, improving the quality and efficiency" in tobacco production, field experiments of flue-cured tobacco transplanting with plastic film marked on hole spacing (T-FMHS) were carried out in 9 of flue-cured tobacco production regions in 2012, by using the hole spacing by marked rope as control (T-RMHS). The results showed that, compared with T-RMHS, the T-FMHS with high degree of standardization, could effectively ensure the transplanting density of 16 500 plants/ha; The labor cost was reduced by 50%, and the tobacco transplanting time was shortened largely. Average yield and income increased by 25.2 kg/ha and 641 yuan/ha, respectively. Those results suggest that, by use of Plastic Film Marked on Hole Spacing in flue-cured tobacco transplanting, the standardization degree of tobacco transplanting and farmer income can increase significantly. This technology will be extended to all of the tobacco production regions in Guizhou province in 2013.