Abstract: In order to investigate the possible difference of seed vigor and seedling of tobacco seed produced in high altitude and low altitude, the seeds of MS Yunyan 85, MS Yunyan 87 and MS K326 produced in low altitude (Banna) and high altitude such as Qujing and Zhaotong were germinated under room temperature (26 ℃) and low temperature (13 ℃), respectively. Comparison of emergence time and seedling time also were conducted in 6 tobacco-plant regions. The results showed that germination rate and germination percentage of seeds were almost the same under room temperature. The seed germination percentage of MS Yunyan 85 and MS Yunyan 87 produced in low altitude were higher than that in high altitude. The seeding time of all seeds was almost the same, which were sown in the same tobacco-plant region in the same year. The seedling time was the same. So the seed vigor and seedling rate of tobacco seed cannot be influenced by the altitude of seed production.