Abstract: To investigate the effect of nicotine on mice swimming tine and physical and chemical indicators of fatigue, SPF Kunming male mice were randomly divided into four groups. Experimental groups were injected subcutaneously nicotine tartrate (Formulated with saline) daily at concentrations of 0.1, 0.5, 1.0 mg/kg. Control group was injected with saline at the concentration of 1.0 mg/kg. After 20 days, mice weight were weighed, the swimming time, urea nitrogen, blood lactate and glycogen were measured. The results showed that 0.5 mg/kg nicotine tartrate could increase swimming time significantly, reduce blood urea concentration and blood lactate glycogen concentration significantly, but could not affect the liver glycogen concentration. The mechanism was speculated that nicotine might stimulate the secretion of hormones and enhances activity in mice.