Abstract: In order to find out the suitable cultivation practices for KRK26, the flue-cured tobacco cultivar introduced from Zimbabwe, in Yichang, Hubei Province, the effects of row spacing, plant spacing and the number of leaves after topping on yield and main economic characters of the flue-cured tobacco variety KRK26 were studied with L₉(3⁴)orthogonal experiments. The results showed that row spacing had a highly significant impact on average price and the percentage of above-average grading leaves. Plant spacing had a highly significant impact on yield, output, proportion of high class leaves and above-average grading leaf percentage, and had a significant impact on the proportion of upper leaves. Leaf number had a highly significant impact on output. The appropriate cultivation measures of KRK26 include 120 cm of row spacing, 55 cm of plant spacing and 21-23 leaves in Yichang city of Hubei Province.