Abstract: In order find out the influence on tobacco growth and possible phytotoxic degrees of 54 herbicides, foliar spray was conducted at the forth leaf stage, sixth leaf stage and before rosette stage to induce phytotoxicity. The results showed that, 40, 41 and 31 of the herbicides caused serious phytotoxicities on tobacco applied at the three developmental stages. Herbicides of the same type showed similar phytotoxic degrees. All herbicides except Aryloxygen-based acid and Cyclohexenone caused various degrees of phytotoxicities on tobacco. 12 types of herbicides, including Phenoxy-carboxylic-acid, sulfonylureas, diphenylethers and etc, could cause serious phytotoxic damages. 18 herbicides were newly discovered to be able to induce serious phytotoxicity on tobacco.