烟草青枯病拮抗菌在有机肥中的定殖效率及田间防治效果

孙立广; 张洪春; 赵秀云; 王瑞; 向必坤; 彭五星; 孙玉晓; 尹忠春; 谭军

doi:10.13496/j.issn.1007-5119.2016.04.009

烟草青枯病拮抗菌在有机肥中的定殖效率及田间防治效果

Colonization Rate of Several Antagonistic Bacteria against Tobacco Bacterial Wilt in Organic Fertilizers and Control Efficacy in Field

摘要

摘要: 为有效防治青枯病，采用多种拮抗菌复配菌剂添加至生物有机肥，施用于烟田，研究其对烟田土壤中有益微生物、病原微生物的影响及对青枯病的控制效果。结果显示，30%茶枯+70%烟草秸秆粉碎物（生物有机肥C）中添加的拮抗菌有较好的定殖率，枯草芽胞杆菌JK-4、解淀粉芽胞杆菌JK-10菌株在生物有机肥C中的定殖率分别达到了945.7%、1970%，链霉菌LC-7菌株在生物有机肥C中定殖率达到496.7%，同时其防治青枯病的效果最好，在烟草旺长期防治效果达到57.40%，在青枯病发病高峰期防治效果为37.11%。

Abstract: For effective prevention and treatment of bacterial wilt, a variety of antagonistic bacterial agents were added to bio-organic fertilizers and applied to tobacco fields. The effects of composite microbial agents added in the bioorganic fertilizers on tobacco beneficial soil microbes, pathogenic microorganisms, and on bacterial wilt control were studied. The results showed that 30%+70% of the dry tea pulverized tobacco stalks (bio-organic fertilizer C) added with antagonistic bacteria had better colonization, with colonization rate of Bacillus subtilis JK-4 and Bacillus amyloliquefaciens strain JK-10 reached 945.7% and 1970% in the biological organic fertilizer C. The Streptomyces strain LC-7 set in bio-organic fertilizer C with a colonization rate of 496.7 percent. The best prevention of bacterial wilt was obtained with this treatment, with a control effect of 57.40% during the fast growing period of tobacco and a control effect of 37.11% at the peak of incidence of bacterial wilt.

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