毁灭炭疽菌诱抗蛋白的分离纯化及对烟草抗病促生作用

田华; 陈光勇; 董瑜; 王静; 王贻鸿; 孔凡玉

doi:10.13496/j.issn.1007-5119.2016.04.012

毁灭炭疽菌诱抗蛋白的分离纯化及对烟草抗病促生作用

Purification of a Protein Elicitor from Colletotrichum destructivum and Its Biological Activity in Inducing Tobacco Resistance and Facilitating Growth

摘要

摘要: 为明确诱抗蛋白能够对烟草产生抗病促生作用，采用加热、离心、硫酸铵分级沉淀、透析和凝胶色谱柱等方法，从毁灭炭疽菌（Colletotrichum destructivum）中分离纯化出一种诱抗蛋白，并采用喷雾接种和摩擦接种的方法研究其预防保护作用。结果表明，经SDS-PAGE显示该诱抗蛋白分子量约为38kD，且目的蛋白存在于Pro-P2-TX组份中；对烟株进行烟草普通花叶病毒病的接种试验，接种3d后，诱抗蛋白处理组烟草叶片枯斑数明显少于清水对照组，对TMV的诱抗效果为73.79%；该诱抗蛋白能够明显促进烟草的生长，诱导处理40d后，烟草的株高、叶宽和茎围与清水对照相比明显增加，其中株高增长72.43%，叶宽和茎围分别增加61.06%和29.05%。因此，试验结果说明毁灭炭疽菌诱抗蛋白具有诱导烟草产生抗病性和促进烟草生长的作用。

Abstract: To functionally investigate the mechanisms of disease resistance and tobacco growth, we have isolated a protein elicitor from mycelium of Colletotrichum destructivum by heating, centrifugation, precipitation, dialysis and gel chromatography column. We then measured the prevention and protection effects of this protein using spray and friction inoculation methods. The results showed that the protein elicitor appeared in Pro-P2-TX and the molecular weight was approximately 38 kD on silver-stained SDS-PAGE. The inhibitory effect to tobacco mosaic virus(TMV)was 73.79%. The protein elicitor could remarkably increase the individual plant height by 72.43%, leaf width by 61.06% and stem circumference by 29.05% respectively. Taken together, the protein elicitor has the role of inducing tobacco resistance and facilitating growth.

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