Abstract: A field experiment was conducted to study the effects of antichor on chlorine absorption and accumulation, yield and quality of flue-cured tobacco. The results indicated that the addition of 5‰ antichor could promote growth and development, and increase dry matter accumulation of tobacco. However, the addition of 10‰ antichor was not beneficial to tobacco growth and dry matter accumulation. Application of antichor did not reduce chlorine content and accumulation in root and stem, but significantly reduced the chlorine content and accumulation in the middle and lower tobacco leaves. The antichor addition significantly increased the distribution of chlorine in root and stem, but significantly reduced the distribution of chlorine in leaf. The soil chlorine content could be ranked as no anticho <5‰ antichor <10‰ antichor treatments. Applying antichor could inhibit tobacco leaf chlorine absorption, but might result in large amount of chlorine residual in the soil. Comprehensive studies showed that the antichor application should be kept at about 5‰ to maintain agronomic traits, economic characters, smoking quality and chlorine reduction of flue-cured tobacco.