Abstract: Solanesol is not only an important intermediate for the synthesis of ubiquinone drugs, such as coenzyme Q10 and vitamin K2, but is also an important precursor of polycyclic aromatic hydrocarbons in cigarette smoke, and has a great influence on the safety of cigarette. In this paper, the solanesol biosynthetic pathways and their key enzyme genes were summarized. It provided useful information for medical value development of tobacco and cultivation of less harmful tobacco varieties.