Abstract: To screen good parents with high neutral aroma component content and best heterosis, 5 genotypes of tobacco were chosen as parents to test by using the half diallel cross model in hybridization experiment. Ten crosses were acquired from 5 tobacco varieties. Total content of neutral aroma and its various components of all parents and cross combinations were determined. Heterosis and combining ability of neutral aroma component content were analyzed. The results showed that 664-01 and Yunyan 87 showed higher neutral aroma content and combining ability, with high GCA values and heritability, therefore they are good parents in breeding that aims at increasing neutral aroma components. Mid-parent heterosis and heterosis of NC 89×664-01 were positive. Considering it's relatively high SCA value, this cross presumably was the best combination to improve the content of neutral aroma. Zhongyan 100×Yunyan 87, Qinyan 96×Yunyan 87 and Zhongyan 100×Qinyan 96 had higher heterosis. Therefore, these cross combinations were of great value in tobacco breeding that aims at improving the neutral aroma content.