Abstract: In order to screen for varieties of green manure in Southern Anhui tobacco areas to promote the tobacco yield and quality, Astragalus sinicus, Medicago falcata and Secal ecerale were planted in tobacco planting soils. Biological traits and main chemical components of green manures were measured before ploughed under the soil. The tobacco agronomic traits, main chemical component, yield and the quality from different green manure treatments were analyzed. The results showed that the yield of dry grass of Secal ecerale was the highest, being 1.97 t/ha, 2.46 and 7.30 times of that of Astragalus sinicus and Medicago falcate respectively. The maximum area of waist leaves of Astragalus sinicus and Secal ecerale treatments were significantly higher than CK and Medicago falcate. The tobacco leaf yield of Medicago falcate and Astragalus sinicus treatments were increased by 9.67% and 3.53%, and economic values were increased by 17.59% and 10.75%, repectively. The harmony of chemical composition of top leaves from the Secal ecerale treatment was better than that from Medicago falcate and Astragalus sinicus. The smoking quality of top leaves from different treatments displayed the same trend. In conclusion, Secal ecerale can be considered as the alternative green manure in Southern Anhui tobacco areas.