以关联分析发掘烟草抗赤星病基因分子标记

朱承广; 任民; 蒋彩虹; 张雨生; 孙明铭; 刘旦; 程立锐; 杨爱国; 王元英

doi:10.13496/j.issn.1007-5119.2017.01.012

以关联分析发掘烟草抗赤星病基因分子标记

Identification of Molecular Markers for Tobacco Brown Spot Resistant Genes through Association Analysis

摘要

摘要: 筛选与抗赤星病位点紧密关联的分子标记，为烟草分子标记辅助育种提供依据。利用404对SSR引物对96份烟草种质材料进行基因组扫描，分析其群体结构，并进行烟草赤星病抗性关联分析。结果表明，在两种不同环境条件下共检测到38个与烟草赤星病抗性显著关联的分子标记（P≤0.01），其中标记PT60236、PT50668、PT50176、PT53770、PT60080和PT50727关联强度较大或在不同环境条件下被重复关联到，分别位于6号、8号、9号、19号、3号和23号烟草连锁群上。关联到的分子标记可以用于烟草抗赤星病育种的辅助选择。

Abstract: In order to screen for SSR markers associated with tobacco brown spot resistant genes and to provide useful information for breeders, 404 SSR markers were used to scan polymorphism among a population containing 96 tobacco germplasms in this study. Population structure was analyzed and association analysis between disease index and SSR markers were performed using TASSEL 2.1. 38 SSR markers were identified as being associated with resistant genes at the level of P≤0.01. The results also indicated that PT60236, PT50668, PT50176, PT53770, PT60080 and PT50727 were tightly associated with resistant genes. These SSR markers were located in the 6th, 8th, 9th, 19th, 3th, 23th tobacco linkage group respectively. The markers identified in this study are useful in molecular marker assisted selection in tobacco breeding programs for brown spot resistance.

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