Abstract: In order to investigate the effect of reducing fertilization with fertigation on growth and physiological characteristics of flue-cured tobacco, an experiment had been designed. In this experiment, the base solid nitrogen fertilizer 75 kg/ha+drip irrigation was used as control (CK), the base solid nitrogen fertilizer 30 kg/ha+drip irrigation was used as treatment one (T1), the base solid nitrogen fertilizer 30 kg/ha+ top dressing nitrogen 15 kg/ha of drip fertilization was used as treatment two (T2), the base solid nitrogen fertilizer 30 kg/ha+ top dressing nitrogen 30 kg/ha of drip fertilization was used as treatment three (T3). The results showed that there was no difference in plant height, maximum leaf area and dry matter accumulation between T3 and CK, at the same time, the above indexes were significantly lower in T1 and T2. For chlorophyll content, CK was the highest, T3 took the second place and the chlorophyll content of T1 was the lowest. The SOD activity, POD activity, CAT activity, and nitrate reductase activity in CK were the highest and decreased slowly. The enzyme activities of T1 were the lowest and decreased more rapidly. The enzyme activities of T2 and T3 were decreased slowly during growth period. By contrast, the SOD activity, POD activity, CAT activity, nitrate reductase activity and the chlorophyll content of flue-cured tobacco were decreased significantly in treatments with reduced fertilization with fertigation. T3 and CK had similar agronomic characters, and drip irrigation was beneficial to the accumulation of dry matter.