烟草疫霉菌拮抗细菌的筛选鉴定及发酵条件优化

李小杰; 李成军; 刘红彦; 邱睿; 赵辉; 陈玉国; 胡亚静; 刘畅; 白静科; 李淑君

doi:10.13496/j.issn.1007-5119.2019.01.009

烟草疫霉菌拮抗细菌的筛选鉴定及发酵条件优化

Screening and Fermentation Condition Optimization for Antagonistic Bacteria to Phytophthora Nicotianae

摘要

摘要: 为了筛选对烟草疫霉菌具有拮抗作用的生防细菌菌株，本研究从烟草黑胫病病圃地采集健康烟株的根际土样，采用对峙培养法筛选得到8株对烟草黑胫病具有拮抗作用的细菌菌株，其中编号为LB-9的菌株对烟草疫霉菌的抑制作用最强且效果稳定，抑菌率为60.6%，且抑菌谱广，对其进行16S rRNA基因序列分析后将该菌株鉴定为多粘芽孢杆菌。采用单因素优化试验初步探究了多粘芽孢杆菌LB-9的最适发酵条件，明确了LB-9的最适发酵培养基、最适发酵温度、最适发酵时间和最适发酵初始pH值，对其生防制剂的开发应用具有重要意义。

Abstract: In order to screen the bacterial strains which have antagonistic effects on Phytophthora nicotianae and provide favorable resources for the biological control of tobacco black shank disease in Henan tobacco areas, the rhizosphere soil samples of healthy tobacco plants were collected from tobacco black shank disease nursery and eight bacterial strains were screened by confrontation culture method. Among them, the strain LB-9 had the strongest and stable inhibitory effect on P. nicotianae with a bacteriostatic rate of 60.6% and a broad spectrum. The strain was identified as Paenibacillus polymyxa by 16S rRNA gene sequencing analysis. The optimum fermentation medium, temperature, time and initial pH value of LB-9 were determined by single factor optimization test which has great significance to the development and application of biocontrol agents.

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