Abstract: In this work, the tobacco-specific nitrosamines (TSNAs) release behavior of reconstituted tobacco particles (RTPs) for heat-not-burn product (HNB) when heated has been investigated, and some influence factors, such as the glycerin content of tobacco material and the heating temperature, have been studied. Besides, the relationship between the release of NNN and NNK has been explored. NNK and NNN release amounts increased with heating temperature, and when the temperature reached 240 ℃, the increment decreased; NAB release amount increased with the increase of temperature; NAT release amount increased with the increase of temperature first, and became stable after the temperature reached 240 ℃. Meanwhile, the addition of glycerin to RTPs can reduce the release of TSNAs to varying degrees. Hence, glycerin content of RTPs and the heating temperature have both been shown to considerably influence the release values of TSNAs, while appropriate glycerin content and relatively low heating temperature could reduce the release of TSNAs efficiently. Besides, when NNN was added to RTPs, NNK release amount increased significantly, so it could be conjectured that the increase of NNN content in RTPs may promote the release of NNK in aerosol.