Abstract: In order to simplify the fertilization of flue-cured tobacco in mountainous regions, a pot experiment was carried out to determine and analyze the effects of different fertilization treatments (blank, no fertilization; CK, fusible tobacco special compound fertilizer; H1 treatment, organic fertilizer:inorganic fertilizer=1:1, H2 treatment, organic fertilizer:inorganic fertilizer=2:1) on dry matter and nutrient accumulation, nutrient distribution ratio, and nutrient utilization ratio of flue-cured tobacco root, stem and leaf. The results showed that compared with the blank, accumulation of dry matter and N, P, K of root, stem, leaf and whole plant was significantly (p ≤ 0.01) increased in the other treatments. Compared with the CK, the K accumulation amount, apparent utilization ratio and economic utilization ratio of H1 treatment were increased significantly (p ≤ 0.01), and that the apparent utilization ratio and economic utilization ratio of H1 treatment were increased 24.85 and 19.16 percentage points, respectively. The accumulation of dry matter and N, P, K of root, stem and leaf of H2 treatment were significantly (p ≤ 0.05 or p ≤ 0.01) increased and the apparent utilization ratios of N, P, K were significantly (p ≤ 0.01) increased, which was 21.15, 6.10 and 24.59 percent higher than the CK, respectively. Simultaneously, the economic utilization ratios of N, P, K of H2 treatment were significantly (p ≤ 0.05 or p ≤ 0.01) increased by 10.97, 3.79 and 18.44 percentage points compared to the CK, respectively. Compared with the CK, the N distribution ratio of leaves was reduced while that of K was increased in organic-inorganic compound fertilizer (H1 and H2). In summary, H2 treatment showed the best performance.