Abstract: In order to understand the variation of main quality traits on middle leaves of different flue-cured tobacco varieties, descriptive statistics and variance analysis were applied to conduct a comparative analysis on 160 raw tobacco samples of the middle leaves of China's seven flue-cured tobacco varieties (Cuibi 1, Honghuadajinyuan, KRK26, Qinyan 96, Yunyan 85, NC55, Longjiang 911). The results showed that:(1) there was significant difference in the appearance quality of the middle leaves from the seven flue-cured tobacco varieties, and the overall variation of the appearance quality of the seven flue-cured tobacco varieties was relatively small. However, both the colors of the middle leaves from the seven varieties after modulation and the oil content of Hongda and KRK26 tobacco showed moderate variation; (2) There was significant difference in the conventional chemical constituents as well as smoke quality among the seven flue-cured tobacco varieties. The chemical constituents of all the varieties and smoke quality of Yunyan 85, Qinyan 96, and Longjiang 911 were all of moderate variation. The variation of smoke quality of Hongda, NC55, Cuibi 1, and KRK26 was small; (3) Yunyan 85 ranked first in the appearance quality, whose comprehensive score was 81.99; Cuibi 1 ranked first in smokequality, whose comprehensive score was 64.64; the main quality traits of the middle leaves from Cuibi 1, Yunyan 85, Hongda, and KRK26 were superior to those of Longjiang 911, Qinyan 96, and NC55.