Abstract: In order to find out the effects of different agronomic measures such as herbicide, mulch and megathermal flame on soil fungal community and function, high-throughput sequencing and FUNGuild were used to analyze the soil fungal community structure and functional groups under different agronomic measures. There were seven treatments, including control (CK; no herbicide, no mulching film and no megathermal flame treatment), apply herbicide + mulching film treatment (T1), apply herbicide treatment (T2), mulching film treatment (T3), mulching film + mid-range firepower flame treatment (T4), mid-range firepower flame treatment (T5) and high-grade firepower flame treatment (T6). The results showed that the soil fungi community was mainly composed of Basidiomycea, Ascomycete and Mortierella. No significant effect on soil α diversity index was observed, but there was significant difference in fungal genus abundance and fungal community structure between different agronomic measures. Compared with the control, the abundance of pathotroph fungi was significantly reduced in the soil of mulching film treatment, mid-range firepower flame treatment and high-range firepower flame treatment, in which the risk of fungal diseases in tobacco decreased. As for the herbicide treatment, the risk of fungal diseases also decreased, but the reduction of saprotroph may lead to inhibited decomposition of soil organic matter.