普通烟草脂氧合酶基因家族鉴定及表达模式分析

李志远; 宋青松; 刘江; 王德勋; 范志勇; 吴新儒; 李晓旭; 王奇; 孙晋浩; 郭存; 晁江涛; 李功博; 刘贯山

doi:10.13496/j.issn.1007-5119.2021.05.014

普通烟草脂氧合酶基因家族鉴定及表达模式分析

Identification and Expression Analysis of the Lipoxygenase Gene Family in Tobacco (Nicotiana tabacum L.)

摘要

摘要: 脂氧合酶（LOX）是脂肪酸代谢途径的关键酶，在植物的生长发育及多种逆境胁迫响应过程中发挥重要作用。本研究对烟草基因组中的LOX家族成员进行鉴定，并利用进化分析、共线性分析和表达分析等方法解析LOX家族成员的潜在生物学功能。结果表明，在普通烟草中共鉴定得到18个LOX基因，其中12个LOX基因被锚定在染色体上。系统进化分析表明，普通烟草中新鉴定的LOX家族成员被分为9-LOX和13-LOX两个亚家族，其中13-LOX又可被分为Type I和Type II两个亚类。共线性分析表明，烟草NtLOX07、NtLOX10基因分别与拟南芥AtLOX05、AtLOX01基因形成同源基因对，并且大部分同源基因之间具有相似的表达模式。表达模式分析发现，普通烟草LOX基因表达具有一定的组织特异性，并且NtLOX06等基因能够被机械损伤和茉莉酸甲脂（MeJA）处理显著诱导。因此，普通烟草LOX家族成员可能在植物胁迫响应过程中发挥着重要的作用。

Abstract: Lipoxygenase (LOX) is a key enzyme in fatty acid metabolism of plant and plays multiple roles in development and stress responses. The identification and analysis of LOX family members in tobacco can provide a theoretical basis for tobacco resistance breeding. In the current study, a total of 18 LOX family members were identified in tobacco, and 12 of those were anchored on chromosomes successfully. The phylogenetic analysis revealed that the newly identified tobacco LOX family members could be divided in 2 subgroups (9-LOX and 13-LOX). And then, LOX members in the 13-LOX subgroup could be classified in two different types (Type I and Type II). The syntenic analysis revealed that there were two homologous gene pairs between Arabidopsis and tobacco, and they shared similar expression patterns. Notably, the expression pattern analysis indicated that the NtLOX genes expression patterns were different in tested tissues, while several NtLOX genes could be induced by wounding and MeJA treatments, such as NtLOX06. Therefore, LOX family members in tobacco may play a significant role in the process of plant stress response.

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