Abstract: The Yunbai 2 is a new burley tobacco variety derived from Kentucky14×Burley64. Traits of this variety are stable in inheritance, and it has more compact plant architecture, trim growth in field and strong growth vigor. Average height, stem girth and internodal length are 152.7 cm, 12.2 cm and 5.0 cm after decapitation, respectively. With 25 of productive leaves, the average length and width of oval lumbar leaf are 73.5 cm and 32.1 cm. Seedling stage of it is 55-60 d and the growing stage about 90 d. The variety matures simultaneously, which result in reaping and air curing easily. The variety has good fertilizer tolerance and adaptation. Yunbai 2 is resistant to TMV and black shank, but sensitive to brown spot. Although the average yield is no more than the control, the Yunbai 2 is superior in all of the other main economic characters, such as output value, average price and the middle and up grade of tobacco leaves, the variety has respectively increased by 4.22%-10.66%, 0.38-0.46 yuan/kg. Not only the more appearance quality, but also the variety has more suitable and harmonious chemical constituents, which aromatic style traits are significant with abundant aroma and good permeability. In the case of internal and external quality, the Yunbai 2 is superior to the control.