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    徐 军, 刘艳华, 任 民, 牟建民, 张兴伟, 陈雅琼, 王志德. 普通烟草种质资源的SSR标记与指纹图谱分析[J]. 中国烟草科学, 2011, 32(2): 62-65. DOI: 10.3969/j.issn.1007-5119.2011.02.014
    引用本文: 徐 军, 刘艳华, 任 民, 牟建民, 张兴伟, 陈雅琼, 王志德. 普通烟草种质资源的SSR标记与指纹图谱分析[J]. 中国烟草科学, 2011, 32(2): 62-65. DOI: 10.3969/j.issn.1007-5119.2011.02.014
    shu
    XU Jun, LIU Yanhua, REN Min, MU Jianmin, ZHANG Xingwei, CHEN Yaqiong, WANG Zhide. SSR Fingerprint Map Analysis of Tobacco Germplasms[J]. CHINESE TOBACCO SCIENCE, 2011, 32(2): 62-65. DOI: 10.3969/j.issn.1007-5119.2011.02.014
    Citation: XU Jun, LIU Yanhua, REN Min, MU Jianmin, ZHANG Xingwei, CHEN Yaqiong, WANG Zhide. SSR Fingerprint Map Analysis of Tobacco Germplasms[J]. CHINESE TOBACCO SCIENCE, 2011, 32(2): 62-65. DOI: 10.3969/j.issn.1007-5119.2011.02.014
    shu

    普通烟草种质资源的SSR标记与指纹图谱分析

    SSR Fingerprint Map Analysis of Tobacco Germplasms

      摘要
    • 摘要: 利用SSR标记构建了烟草核心种质中80份普通烟草种质的指纹图谱。从286对SSR引物中筛选出8对多态性较好的引物，对这些烟草种质进行分析，共检测到 85个多态性位点。研究表明，这8对多态性SSR引物可以将80份烟草种质完全区分开，每份种质都有各自独特的指纹图谱，表明SSR标记完全适用于普通烟草种质鉴定和指纹图谱构建工作。

       

      Abstract: DNA fingerprint maps of 80 tobacco germplasms were constructed by SSR molecular maker. 8 pairs of primers were selected from 286 pairs and a total of 85 polymorphic SSR bands were detected. The 80 tobacco germplasm could be absolutely identified by these 8 pairs of SSR primer, every tobacco germplasm had a unique fingerprint map, which demonstrates that SSR fingerprint map is suitable for tobacco cultivar identification.

       

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