Abstract: Five different kinds of representative organic fertilizer samples collected from Yishui, Shandong province were studied. The contents of organic matters, nitrogen, total sugar, crude fat and 17 kinds of common amino acids were determined after 14 weeks’ situ cultivation and waterlogged cultivation. The result indicates that application of manure to brown soil increased the contents of organic matters, nitrogen and amino acids. After situ cultivation, samples of total sugar and crude fat content were higher than that of the control; after waterlogged cultivation, in each treatment total sugar content were lower than that of the control; Join straw and chicken manure processing crude fat content were lower than controls, in the other treatment that were higher than the control. In situ cultivation, pig manure had the biggest influence on total amino acid, straw and chicken manure, ryegrass took the second place, soybean cake the last; In waterlogged cultivation, the order was straw > ryegrass > pig manure > soybean cake > chicken manure. Organic fertilizer had obvious impact on the soil nutrient content.