一个与净叶黄抗赤星病基因紧密连锁的SSR标记

A SSR Marker Tightly Linked to the Resistant Gene of Jingyehuang on Tobacco Brown Spot

摘要: 为了在分子水平上弄清烟草赤星病抗性的遗传规律,并进行遗传定位,以抗赤星病品种净叶黄和感赤星病品种NC82为亲本,构建了F₁、F₂、BC₁ 代群体。通过对该群体进行赤星病接种鉴定和抗性遗传分析,发现净叶黄对赤星病的抗性由显性多基因控制。通过分子标记群体扩增,在第M号连锁群上,筛选到一个与净叶黄的赤星病抗性基因紧密连锁的SSR标记,它与抗性基因间的遗传距离为4 cM。该标记可用于抗赤星病育种的辅助选择。

Abstract: In order to study the tobacco brown spot disease resistance inheritance, and genetic mapping, F₂ and BC₁ populations from a cross between Tobacco Brown Spot resistant variety Jingyehuang and susceptible variety NC82 were developed. Values of disease index under artificial inoculations showed that the resistant trait of Jing-yehuang was fitted additive-dominance model. Meanwhile, a further molecular study was carried out using SSR markers based on a former genetic mapping, and a SSR marker which was tightly linked to the tobacco brown spot resistant gene located on M linkage groups with distance of 4 cM was obtained. This marker provides useful information for molecular marker assistant selection of brown spot resistance in breeding program of tobacco.