Abstract: In order to further explore the relationship between the sugar content in flue-cured tobacco in Dali and smoking quality, 50 samples of flue-cured tobacco K326 from four Dali tobacco-growing areas were taken. By means of fitting curve estimation and grey correlation analysis, the relationship between the sugar content and smoking quality was investigated. The results showed that: tobacco K326 had slightly high total sugar and reducing sugar contents, but the ratio of the reducing sugar to the total sugar was slightly low. In dali, the optimal total sugar content in K326 was 28%-32%, and reducing sugar content 23%-27%. The sugar content in K326 in Dali had closely relationship with the smoking quality, the ratio of reducing sugar to total sugar was most closely correlated with smoking quality, which worth further studying.