Abstract: This study investigated soil available nitrogen, the growth of tobacco plants and the yield and quality of tobacco leaves under the condition of long term fertilization. The results showed that application of organic fertilizer could promote the uptake and accumulation of nutrient in tobacco plant, and improve the quality of tobacco leaves. Organic fertilizer with rice straw application could improve the soil micro-environment, the rate and efficiency of nutrient transformation, dynamics of soil inorganic nitrogen process more in line with the tobacco plant nitrogen accumulation. The results also indicated that soil nitrate changed most among soil available nitrogen, and ammonia content was low and changed little.