Abstract: This study aims to estimate the chlorophyll content and leaf area index (LAI) of tobacco with rapid, harmless and accurate methods to obtain the information on its growth vigor and to provide technological support for the management of cultivation. Under different fertilization conditions, we analyzed the spectral characteristics of tobacco canopy treated in different ways through ASD Field HH spectrometer, and studied the correlation of hyperspectral characteristics with chlorophyll content and LAI in tobacco canopy at nitrogen fertilizer of different levels through relevant statistical methods (such as correlation analysis and regression etc.). Under different fertilization conditions, the spectral reflectance of tobacco canopy was significantly different, normalized different vegetation index (NDVI (green) was significantly related to chlorophyll content and LAI, and the regression coefficient of established regression equation, associated probability and determination coefficient were all reaching to significant level. Therefore, the growth state of tobacco could be quickly investigated through this established estimation model, so as to control the fertilization of tobacco field and take suitable planting management measures on time.