Abstract: To clarify the application effect of plant growth regulator Tianda-2116 in tobacco production, the late flower tobacco variety K326 was used as test material to investigate the effects of Tianda-2116 on agronomic and economic traits, disease resistance, yield and main chemical compositions of flue-cured tobacco under field condition through random block design. The results showed that spraying Tianda-2116 improved tobacco growth, agronomic and economic traits of tobacco leaves, increased tobacco leaf yield, and improved disease resistance. When Tianda-2116 was applied at 500 times solution using a knapsack sprayer, the leaves had more unisonous chemical compositions and better economic traits. The yield and production value of tobacco leaves increased 243.1 kg/ha and 3719.7 yuan/ha as compared with CK, respectively. Differences were statistically significant between treatment and CK. The proportion of upper and middle tobacco leaves was 7% more than those of CK. These results indicated that spraying Tianda-2116 could increase yield and quality of tobacco leaves.