TMV拮抗细菌A3的培养基优化研究

战徊旭; 申莉莉; 韩志忠; 杨金广; 钱玉梅; 王盼; 王凤龙

doi:10.3969/j.issn.1007-5119.2013.02.009

TMV拮抗细菌A3的培养基优化研究

Medium Optimization of Antagonistic Bacteria A3 Strain Against TMV

摘要

摘要: 利用单因素试验结合正交试验的方法,以菌体生长量(OD₆₀₀值)作为测定指标,对TMV拮抗细菌恶臭假单胞杆菌A3的培养基进行优化,系统研究了碳源、氮源、无机盐的发酵结果.试验结果表明,碳源为麦麸,氮源为酵母浸粉,无机盐为CaCl₂、CoCl₂、MgSO₄、FeSO₄、KH₂PO₄时有利于A3的生长.7因素3水平正交试验结果表明,培养基的最优组合为麦麸15 g,酵母浸粉15 g,CaCl₂ 2.5 g,CoCl₂ 0.5 g,MgSO₄ 0.8 g,FeSO₄ 0.5 g,KH₂PO₄ 0.8 g,蒸馏水1000 mL,pH 7.0,在此条件下拮抗细菌活菌数可达3.234×10¹² cfu/mL,比NB培养基中活菌数提高一个数量级.

Abstract: The experiments with one factor and orthogonal design were conducted to optimize medium of antagonistic bacteria A3 strain against TMV (Pseudomonas putida) with mass growth (OD₆₀₀ value) as the index. A systematic study of carbon sources, nitrogen sources and inorganic chemicals was carried out. Carbon source “wheat bran”, nitrogen source “yeast extract powder” and inorganic chemical “CaCl₂, CoCl₂, MgSO₄, FeSO₄, KH₂PO₄”, were beneficial to the growth of A3. The results of L₁₈(3⁷) orthogonal tests showed that optimal medium menu included 15g wheat bran,15g yeast extract powder, 2.5g CaCl₂, 0.5g CoCl₂, 0.8g MgSO₄, 0.5g FeSO₄, 0.8g KH₂PO₄ and 1000mL distilled water, pH 7.0. The number of live bacteria could be 3.234×10¹² cfu/mL in the optimized medium and the number increased by one orders of magnitude than original NB medium.

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