Abstract: In order to evaluate the bio-environmental safety of three kinds of herbicides, which were often applied to tobacco soil, an analytical method was established to simultaneously determine the residues of haloxyfop-p-methyl, fluazifop-p-butyl and quizafop-p-ethyl in soil and water in tobacco planting field by high performance liquid chromatography. The residues in water was extracted with mixed solution of methylene chloride-acetone (20:1, v:v). The soil was extracted with acetone and the extract was cleaned up by a Florisil chromatography column. The quantification was performed by external standard. The linearity was satisfied (r>0.99) in concentration range of 0.0050-5.0 mg/L. The average recoveries of this three herbicides varied from 77.5% to 110.3% with relative standard deviation of 1.6%-10.2%, when three kinds of herbicides were spiked at 0.050-1.0 mg/kg level in soil. The average recoveries of the three herbicides varied from 88.9% to 101.2%, with relative standard deviation of 0.9%-8.2%, when three kinds of herbicides were spiked at 0.0050-1.0 mg/L level in water. The method in this study could meet the requirements of pesticide residue analysis criteria.