Abstract: In July of 2012, the diseased tobacco samples were collected from the tobacco growing areas near the tomato plantations in which tomato yellow leaf curl virus disease and whitefly Bemisia tabaci occurred very seriously. The DNA of the samples were tested by PCR using degenerate primers (PA/PB) of geminivirus. The fragments of expected size (0.5 kb) were obtained and sequenced. The blast result showed the causal agent of the disease was a tomato yellow leaf curl virus isolate (TYLCV-SDTT). Then complete genome sequence of the isolate was amplified. Analysis of similarity between the obtained complete genome sequence and those deposited in the GenBank database was performed. The results shows that TYLCV-SDTT (GenBank accession JX856172) shared homology of 99% with TYLCV tomato isolate (JF414236) in Shandong province, and belonged to the same large branch with other TYLCV isolates belonging to Israel strain of TYLCV (TYLCV-IL). This is the first report that Tomato yellow leaf curl virus infects tobacco under natural conditions in China.