Abstract: The experiment was carried out to form formula with eleven kinds of mediums, including charred rice husk, expanding perlite, red mud, peat, cinders coal, discard germ stick, charred discard germ stick, charred tobacco stem, charred rice straw, compost and seedcake fertilizer. Study on the effect of different medium on the growth of tobacco seedling by checking against the currently tobacco normal seedling formula, to be screened local materials, low-cost, environment -friendly( non-peat) and cultivation of good results matrix formula. The aim is to reduce the cost of seedling and make the development of factory seedling in the region possible. The results showed that when the proportion of charred rice husk and red soil was 7:3, 6:4(proportion of bulk), its seedlings growth of matrix formulation was better, and it can be popularized in the production practice of flue-cured tobacco seedling.