高效液相色谱法同时测定烟草中吡虫啉、多菌灵、甲基硫菌灵的残留量

Determination of Imidacloprid, Carbendazim and Thiophanate-methyl in Tobacco by High Performance Liquid Chromatography

摘要: 用固相萃取-高效液相色谱法对烟草中吡虫啉（imidacloprid）、多菌灵（carbendazim）、甲基硫菌灵（thiophanate-methyl）3种农药同时进行残留分析。用丙酮/水（1:1，V/V）提取，二氯甲烷萃取，紫外检测器分析，检测波长为270 nm，流动相为甲醇/水（60:40，V/V）。试验结果表明，方法回收率吡虫啉为91.9%～97.1%，相对标准偏差为3.97%～10.01%；多菌灵为92.7%～99.9%，相对标准偏差为3.15%～5.37%；甲基硫菌灵为87.4%～95.3%，相对标准偏差为3.05%～4.61%。该方法操作简便，分离效果好，准确度和精密度均达到定量分析要求。

Abstract: The residues of three pesticides (imidacloprid, carbendazim, thiophanate-methyl) in tobacco leaves were analyzed by using high performance liquid chromatography. The samples were distilled by acetone/water (v/v=1:1), extracted by dichloromethane and analyzed by using UV detector at 270nm. The liquid phase was methanol/ water(60:40). The analytical results showed that the recovery ratios of imidacloprid was 91.9%-97.1% and the relative standard deviations was 3.97%-10.01%, carbendazim was 92.7%-99.9% and the relative standard deviations was 3.15%-5.37%, thiophanate-methyl was 87.4%-95.3% and the relative standard deviations was 3.05%-4.61%.This method showed simple, good separation effect and high accuracy and precision met the demand of quantitative analysis.