烟草中杀虫单残留分析方法研究

Analysis Method of Monosultap Residue in Tobacco Leaves

摘要: 对烟叶中农药杀虫单进行残留分析方法研究。试验采用HCl溶液提取，在碱性条件下衍生为沙蚕毒素，经气相色谱（FPD-S）测定沙蚕毒素含量，推知样品中杀虫单残留量。通过pH、水浴温度、水浴时间等外界条件对衍生化效率的影响试验，确立杀虫单残留分析中关键步骤衍生化反应的最佳条件为pH 9.0～9.5、70 ℃水浴2 h，在此条件下，烟叶中杀虫单平均添加回收率87.74%～91.44%，相对标准偏差3.69%～5.73%。结果表明，该方法灵敏度和回收率均符合农药残留分析要求。

Abstract: Monocular residues in tobacco leaves were analyzed by high performance gas chromatography. In this method residues were extracted with HCL and transformed into Nereistoxin in alkali liquid, then calculated the content of Monocular in tobacco. This study confirmed the optimum condition of transform efficiency of Nereistoxin through changing the pH, temperature and time. It demonstrated that the optimum condition was pH 9.0-9.5 and water bath temperature 70 ℃ for 2 hours. In this condition, recoveries in tobacco leaves was 87.74%-91.44%, and relative standard deviations was 3.69%-5.73%. This method meets the requirement in term of sensitivity and recovery.