Abstract: In order to investigate the quality characteristics of tobacco in Guizhou and produce high quality tobacco leaf, the 4 representative tobacco production areas with different altitudes were selected to identify the quality characteristics of tobacco leaf. The result showed that quality style was different obviously with the same appearance quality, so the various tobacco characteristics come into being. The flavor style was from middle scent close to faint one to middle scent and to faint scent close to middle one with the altitude from middle-lower one to middle one to high one. The nicotine content increased and the sugar decreased with the altitude from low to high, but the others no change. The total aroma constituents, half volatilization components and various aroma constituents were the highest in middle altitude that others, while the total various aroma constituents in middle leaf was enhance with altitude increasing and the topping stages was higher than middle one. The changing of aroma constituents with altitude was the characteristic of the Guizhou tobacco.