Abstract: The relationships between aroma and taste at different position of tobacco leaves and altitude,latitude, longitude in Yunnan was analyzed. The results showed that altitude is the main factor effecting ash color of lower leaf, volume of aroma and irritancy of cutters, and latitude is the main factor impacting the flavor notes, mouth feeling and integrated score of cutters, longitude has great influence on volume of aroma, taste concentration, ash color and integrated score of lower leaf, and it also has the influence on the quality of aroma,taste concentration, irritancy and offensive taste of cutters. The canonical correlation analyses showed that longitude and altitude are the main factors influencing the aroma and taste of lower leaf and cutters. The integrated score and volume of aroma is mainly impacted by altitude and longitude.