蒸汽对烟草漂浮苗干根中烟草花叶病毒的失活效果

The Inactivation Effects of Steam to Tobacco Mosaic Virus in Dry Roots of Tobacco Float Seedling

摘要: 为了评价蒸汽用于烟草漂浮育苗旧苗盘残留物中烟草花叶病毒（Tobacco mosaic virus, TMV）的消毒效果，采用枯斑寄主和K326接种方法，测定了蒸汽对烟草漂浮苗花叶病株干根中TMV的失活效果。结果表明，96℃蒸汽蒸3 min～60 min，对病苗干根中TMV失活效果高，可达99.9%。血清学检测结果表明，蒸汽消毒3 min能有效破坏病根样品中TMV抗原。推荐旧苗盘TMV蒸汽消毒方法为：苗盘润湿后96℃蒸汽保持不少于3 min。但在96℃蒸汽中(玉溪，常压蒸汽)保持5 min后，普通聚苯乙烯泡沫苗盘在冷却过程中变形。

Abstract: In order to evaluate inactivation effects of steam to Tobacco mosaic virus (TMV) in residue of float tray, The inactivation effects to TMV in dry roots of tobacco float seedling were evaluated by local lesion assay and inoculation to tobacco variety K326. 96℃ steam keep 3 minutes to 60 minutes could highly inactivated TMV in dry roots of tobacco float seedling, which inactivation ratio are 99.9% to 100%. TAS-ELISA and TMV-Imunostrip test show that steam could fully destroy TMV antigen in dry tobacco seedling roots. 96℃ steam keep not less than 3 minutes is recommend to apply in used float tray for TMV disinfection. But foamed polystyrene plastic trays became distortion after treated at 96℃ steam for 5 minutes.